Dokumentation 26

Distel JW, Hatton JF, Gillespie MJ: Biofilm formation in medicated root canals
Graduate Endodontics Program, Saint Louis University Health Sciences Center,
Missouri 63103, USA. J Endod 2002 Oct;28(10):689-93 

Die Autoren weisen Biofilmbildung trotz Medikation mit Calcium-Hexbroxid in mit E. faecalis infizierten, extrahierten Zähnen nach.

The hypothesis that Enterococcus faecalis resists common intracanal
medications by forming biofilms was tested. E. faecalis colonization of 46
extracted, medicated roots was observed with scanning electron microscopy
(SEM) and scanning confocal laser microscopy. SEM detected colonization of
root canals medicated with calcium hydroxide points and the positive control
within 2 days. SEM detected biofilms in canals medicated with calcium
hydroxide paste in an average of 77 days. Scanning confocal laser microscopy
analysis of two calcium hydroxide paste medicated roots showed viable
colonies forming in a root canal infected for 86 days, whereas in a canal
infected for 160 days, a mushroom-shape typical of a biofilm was observed.
Analysis by sodium dodecyl sulfate polyacrylamide gel electrophoresis showed
no differences between the protein profiles of bacteria in free-floating
(planktonic) and inoculum cultures. Analysis of biofilm bacteria was
inconclusive. These observations support potential E. faecalis biofilm
formation in vivo in medicated root canals.

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