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Dokumentation 37

Gomes BPFA, Sauza SFC, Ferraz CCR, Teixeira FB, Zaia AA, Valdrighi L, Souza-Filho FJ. Effectiveness of 2% chlorhexidine gel and calcium hydroxide against Enterococcus faecalis in bovine root dentine in vitro. International Endodontic Journal, 36, 267-275, 2003.

Aim To evaluate the effectiveness of 2% chlorhexidine gluconate gel and calcium hydroxide (Ca(OH)2) as intracanal medicaments against Enterococcus faecalis.

Methodology One hundred and eighty dentine tubes prepared from intact freshly extracted bovine maxillary central incisors were infected in vitro for 7 days with E. faecalis. The specimens were divided into four groups, according to the intracanal medicament used, as follows: Group 1: 2% chlorhexidine gluconate gel; Group 2: calcium hydroxide in a viscous vehicle (polyethyleneglycol 400); Group 3: 2% chlorhexidine gluconate gel + calcium hydroxide and Group 4: Brain Heart Infusion (BHI) broth (control group). The medicaments were placed into the canal lumen and left there for experimental times of 1, 2, 7, 15 and 30 days. After each period, irrigation with sterile saline to remove the medicament was performed and the canals were dried with sterile paper points. Dentine chips were removed from the canals with sequential sterile round burs at low speed. The samples obtained with each bur were immediately collected in separate test tubes containing BHI broth. The tubes were incubated at 37°C and daily observed for microbial growth, visualized by the medium turbidity.

Results Chlorhexidine gel alone completely inhibited the growth of E. faecalis after 1, 2, 7 and 15 days. Calcium hydroxide allowed microbial growth at all experimental times. The combination of chlorhexidine and Ca(OH)2 was effective after 1 and 2 days demonstrating 100% antibacterial action; however, its antibacterial activity reduced between 7 and 15 days.

Conclusions Under the conditions of this study, it can be concluded that 2% chlorhexidine gel alone was more effective against E. faecalis than calcium hydroxide (P < 0.05). However, its antibacterial activity depended on how long it remained inside the root canal.

 

Die Autoren untersuchen die Wiksamkeit von CHX und Ca(OH)2 auf E. Faecalis an 180 infizierten Dentinzylindern von Rinderzähnen. Während CHX-Gel das Wachstum nach 1, 2, 7 und 15 Tagen vollständig verhinderte, erlaubte Ca(OH)2 Keimwachstum zu allen Zeiten des Experiments. Eine Mischung aus CHX-Gel und Ca(OH)2 war zwar nach 1 und 2 Tagen 100%ig wirksam, die antimikrobielle Aktivität ließ jedoch zwischen dem 7. und dem 15. Tag nach.

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