Dokumentation 57
Assessment
of periradicular microbiota by DNA-DNA hybridisation
Sunde
PT, Tronstad
L, Eribe
ER, Lind
PO, Olsen
I., Microbiology
(2003), 149,
1095–1102
Department of Oral Biology, Faculty of Dentistry, University of Oslo, Oslo,
Norway.
In the present study the "checkerboard" DNA-DNA hybridization
technique was used to identify bacteria in periapical endodontic lesions of
asymptomatic teeth. Thirty-four patients with root-filled teeth and apical
periodontitis were divided into two groups, each containing 17 patients. In
Group 1, a marginal incision was performed during surgery to expose the lesion,
and in Group 2, a submarginal incision was applied. The gingiva and mucosa were
swabbed with an 0.2% chlorhexidine gluconate solution prior to surgery.
Bacterial DNA was identified in all samples from the two groups using 40
different whole genomic probes. The mean number (+/- SD) of species detected was
33.7 +/- 3.3 in Group 1 and 21.3 +/- 6.3 in Group 2 (P < 0.001). The majority
of the probe-detected bacteria were present in more lesions from Group 1 than
from Group 2. The differences were most notable for Campylobacter gracilis,
Porphyromonas endodontalis, Propionibacterium acnes, Capnocytophaga gingivalis,
Fusobacterium nucleatum ssp. nucleatum, Fusobacterium nucleatum ssp. polymorphum,
Prevotella intermedia, Treponema denticola, Streptococcus constellatus and
Actinomyces naeslundii I. Bacterial species such as Actinobacillus
actinomycetemcomitans and Bacteroides forsythus were detected in more than 60%
of the lesions from both groups. Also, P. endodontalis was abundant in
periapical tissue. The data supported the idea that following a marginal
incision, bacteria from the periodontal pocket might reach the underlying
tissues by surgeon-released bacteremia. The study provided solid evidence that
bacteria invade the periapical tissue of asymptomatic teeth with apical
periodontitis. The detection of much more bacteria with the "checkerboard"
DNA-DNA hybridization method than has previously been recovered by anaerobic
culture indicated that the endodontic (and periodontal) microfloras should be
redefined using molecular methods.
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